4:00-4:30 Welcome

4:30-5:30 Bert La Du Memorial Lecture: Dan S. Tawfik
Weizmann Institute of Science, Rehovot, Israel
Structure, mechanism, and evolutionary origins of the mammalian serum paraxonases

Oral Session I: Structure and Biochemistry
Chairs: Tom Magliery and Dan Tawfik

9:30-9:55 Patricia Babbitt and Michael Hicks
University of California, San Francisco, USA
A large-scale view of the Nucleophilic Attack 6-bladed β-Propeller (N6P) Superfamily provides a context for interpretation of linked sequence, structural, and functional features of the paraoxonases

9:55-10:10 Moshe Ben-David, Mikael Elias, Israel Silman, Joel Sussman and Dan Tawfik
Weizmann Institute of Science, Rehovot, Israel
Structural analysis of WT rePON1, its mutants and complexes reveals insights into the binding mode and catalysis of PON1's native and promiscuous substrates

10:10-10:35 Thomas J. Magliery
The Ohio State University, Columbus, USA
Tinkering with paraoxonase-1: insights into physical properties, specificity and mechanism

10:35-10:50 Brian Bahnson
University of Delaware, Newark, USA
Structural and Kinetic Insights into the Gluconolactonase Mechanism of Human Senescence Marker Protein 30

10:50-11:05 Break

11:05-11:20 Yushu Chen, Zeynep Altun and Martin Chalfie
Columbia University, New York, New York, USA
C. elegans paraoxonase-like proteins regulate the transport and activity of DEG/ENaC channels

11:20-11:45 Richard James and Sara Deakin
University Hospital, Geneva, Switzerland
Exploiting PON1 to modulate HDL functionality

11:45-12:00 Xiaodong Gu, Zhiping Wu, Matt Wagner, Joe Didonato and Stanley Hazen
Lerner Research Institute, Cleveland Clinic, Ohio, USA
Anchoring of PON1 to HDL: Insights gained using novel photoactivatable lipid probes, hydrogen-deuterium exchange, and functional studies with related site-specific mutants

Oral Session II: Toxicology
Chairs: Clement Furlong and Betzabet Quintanilla-Vega

1:00-1:25 Nina Holland, Karen Huen, Kim Harley, Lisa Barcellos, Asa Bradman and Brenda Eskenazi
University of California, Berkeley, USA
Genetic and Epigenetic Effects of PON1 on Health Outcomes in Children

1:25-1:40 Betzabet Quintanilla-Vega
Cinvestav-IPN, Mexico
PON1 genetic polymorphisms relationship with organophosphate insecticide susceptibility in Mexican populations

1:40-2:05 Gennaro Giordano, Lucio G Costa, Clement Furlong and Toby Cole
University of Washington, Seattle, USA
Paraoxonase 2 Exerts Neuroprotection in Mouse Neurons and Astrocytes

2:05-2:20 Judit Marsillach, Edward J Hsieh, Rebecca J Richter, Michael M MacCoss, Michael H Paulsen, Christopher D Simpson and Clement E Furlong
University of Washington, Seattle, USA
Paraoxonase-1 and butyrylcholinesterase: biomarkers of sensitivity and exposure to organophosphates in agricultural workers. Proteomics versus enzymatic analyses

2:20-2:35 Break

2:35-2:50 Ramachandra Naik, Shoshana Barnoy, Brandon Pybus, Victor Melendez, Thomas Palys and Ashima Saxena
Walter Reed Army Institute of Research, Silver Spring, Maryland, USA
Paraoxonases 1 and 2 inhibit biofilm formation by Acinetobacter baumannii

2:50-3:05 Alexandros Tselepis, Maria Tsoumani, Kallirroi Kalantzi and Ioannis Goudevenos
University of Ioannina, Greece
Association of serum HDL and Paraoxonase-1 levels with platelet reactivity in patients with acute coronary syndromes treated with clopidogrel

3:05-3:20 Jean-Luc Reny, Christophe Combescure, Youssef Daali and Pierre Fontana
University Hospital, Geneva, Switzerland
Influence of the paraoxonase-1 Q192R genetic variant on clopidogrel responsiveness and recurrent cardiovascular events: a systematic review and meta-analysis

Discussion Session: The Role of Paraoxonase-1 in Clopidogrel Metabolism
Chairs: Jordi Camps and Clement Furlong

3:30-3:45 Opening comments from Jordi Camps (University Hospital Sant Joan, Reus, Spain) and Clement Furlong (University of Washington, Seattle, USA)

3:45-4:00 Open discussion led by Jordi Camps

Evening at leisure in Columbus

Option 1: Leisure time in the nearby Shorth North arts district of restaurants, bars, coffee shops, galleries and boutiques. Transportation will be provided.

Option 2: Columbus Clippers minor league baseball game against the Louisville Bats, 7:05 pm at Huntington Park downtown. It's Sugardale Dime-A-Dog night--hot dogs are only 10 cents all night! Transportation and tickets will be provided. You must select this option during registration to attend.

Oral Session III: Cardiovascular Health
Chairs: Stanley Hazen and Diana Shih

9:30-9:55 Wilson Tang, Hooman Allayee and Stanley Hazen
Cleveland Clinic, Ohio, USA
Clinical and Genetic Association of Serum Paraoxonase and Arylesterase Activities with Cardiovascular Risk

9:55-10:10 Alejandro Gugliucci, Kazuhiko Kotani and Russell Caccavello
University of Touro, Vallejo, California, USA
The ratio paraoxonase 1 activity in small vs large HDL subclasses and their apolipoprotein composition as a window to functional assessment of HDL in atherogenesis

10:10-10:35 György Paragh, Mariann Harangi, Krisztina Gaál, Hajnalka Lőrincz, Mónika Katkó and Ildiko Seres
University of Debrecen, Hungary
Human paraoxonase-1 in systemic autoimmune diseases: Current opinion and future directions

10:35-10:50 Janice Chambers, Howard Chambers, R. Hunter Coombes, J. Allen Crow, Marybeth Dail, Kimberly Davis, Paul Eden, Chiquita McDaniel, Edward Meek and Robert Wills
Mississippi State University, USA
Associations of PON1 activities and genotypes with cardiovascular disease and type 2 diabetes and race

10:50-11:05 Break

11:05-11:20 Ildiko Seres, Mariann Harangi, Mónika Katkó, Hajnalka Lőrincz, Sándor Somodi, Peter Fülöp and György Paragh
University of Debrecen, Hungary
Relationship of chemerin to serum paraoxonase-1 activity in obese non-diabetic patients

11:20-11:45 Diana Shih
University of California, Los Angeles, USA
PON3 deficiency leads to decreased mitochondrial function and increased susceptibility to obesity, atherosclerosis, and gallstone disease

11:45-12:00 Asokan Devarajan, Noam Bourquard, Feng Gao, Ekambaram Ganapathy, Jitendra Verma and Srinivasa Reddy
University of California, Los Angeles, USA
Role of PON2 in innate immune response in an acute infection model

Oral Session IV: Biology, Inflammation and Other Disease
Chairs: Michael Aviram and Dragomir Draganov

1:00-1:25 Michael Aviram
Technion Faculty of Medicine, Rambam Medical Center, Haifa, Israel
HDL-associated Paraoxonase1 (PON1) inhibits oxidative stress and attenuate atherosclerosis development

1:25-1:40 Philip Connelly, Hang Yang and Graham Maguire
Keenan Research Centre, St. Michael's Hospital, Toronto, Ontario, Canada
Hemoglobin and hemin accounts for the oxidative activity of mouse plasma and is increased in paraoxonase 1 knockout mice on a high fat diet

1:40-2:05 Gordon Smith
Cambridge University, United Kingdom
Paraoxonase 3: A potential antioxidant therapy for the preterm human neonate

2:05-2:20 Stella Gagliardi, Kenneth Abel, Pamela Milani, John Cashman and Cristina Cereda
IRCCS Neurological Institute C. Mondino, Pavia, Italy
Regulation of FMO and PON detoxication systems in ALS human tissues

2:20-2:35 Break

2:35-2:50 Anabel Garcia-Heredia, Elisabeth Morin-Kensicki, Robert Mohney, Anna Rull, Alba Folch García, Raúl Beltrán-Debón, Gerard Aragonès, M. Carmen Tormos, Bharti Mackness, Michael Mackness, Diana M. Shih, Jorge Joven, Guillermo Sáez and Jordi Camps
University Hospital Sant Joan, Reus, Spain
Hepatic steatosis and increased susceptibility to liver cancer are present in paraoxonase-1 deficient mice given a high-fat, high-cholesterol diet. A metabolomic approach

2:50-3:15 Sven Horke
University Medical Center, Mainz, Germany
PON2 and PON3 as Central Modulators of Stress and Survival Pathways

3:15-3:30 Mira Rosenblat, Nina Volkova and Michael Aviram
Rambam Medical Center, Haifa, Israel
Triglyceride Accumulation in Macrophages Upregulates Paraoxonase 2 (PON2) Expression via JNK/c-Jun/AP-1 signaling pathway activation

Oral Session V: Chemical Defense
Chairs: David Lenz and Daniel Rochu

8:30-8:55 Julien Hiblot, Guillaume Gotthard, Mikael Elias, Patrick Masson and Eric Chabriere
Laboratorie URMITE, Faculté de Medecine, Marseille, France
Structural Biology contributions in the understanding and increase of phosphotriesterase activities

8:55-9:20 Robert Williams and Marc-Michael Blum
Los Alamos National Laboratory, New Mexico, USA
DFPase and PON1 - Twins or just siblings? Comparison of structure and mechanism of two similar phosphotriesterases

9:20-9:45 Franz Worek and Horst Thiermann
Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany
The use of human paraoxonase (hPON1) in poisoning by organophosphorus compounds – A medical perspective

9:45-10:00 Break

10:00-10:25 Moshe Goldsmith, Yacov Ashani, Yair Simo, Tapesh Kumar Tyagi, Moshe Ben David, Haim Leader, Israel Silman, Joel Sussman and Dan Tawfik
Weizmann Institute of Science, Rehovot, Israel
Directed evolution of PON1 for G and V-type nerve agent hydrolysis

10:25-10:40 Tamara C. Otto, Alexandria R. Will-Cole, Melanie G. Kirkpatrick and Douglas M. Cerasoli
U.S. Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland, USA
Analysis of In Vitro Interactions Between Conventional Therapeutic Organophosphorus Drug Treatments and Variants of Paraoxonase-1

10:40-10:55 Christopher Hadad
The Ohio State University, Columbus, USA
A pharmacophore model of human paraoxonase (PON1) for the hydrolysis of organophosphorus compounds

10:55-11:10 Manojkumar Valiyaveettil, Yonas Alamneh, Bhupendra Doctor and Madhusoodana Nambiar
Walter Redd Army Institute of Research, Silver Spring, Maryland, USA
Prospects of PON1 as a medical countermeasure against nerve agent toxicity